![]() The assay was tested using a 649-member panel of specimens from diverse global locales, 13 HIV-1 seroconversion panels, a panel representing seven of the primary HIV-1 subtypes, an HIV-2 panel, an HIV-1/HIV-2 mixed panel, and 100 prospectively tested specimens. In order to maximize HIV-specific antibody capture, multibranched peptides (MBP) for both HIV-1 and HIV-2 ( 22) were evaluated for use in a single assay that could detect and differentiate HIV infections. The purpose of this study was to develop a quantifiable lateral-flow test for the detection and differentiation of antibodies to HIV-1 and HIV-2 using magnetic-bead markers (magnetic immunochromatography test ). Thus, detection of antibodies generated to a variety of HIV subtypes might be improved through the use of a broader antigenic mix (chimeric recombinant proteins or synthetic peptides) and/or a more effective antigenic presentation (multibranched peptides), both of which have proven useful in diagnostic assays for HIV and other infectious agents ( 13, 15, 19, 22). Furthermore, the development of assays for HIV incidence determinations has shown that the immune responses to subtype B antigens are not equivalent across HIV subtypes ( 21) and that multisubtype antigens are more effective at establishing comparable incidence measurements in international cross-sectional surveys. However, how these assays perform during early seroconversion with non-subtype B infections has not been assessed, since panels for other subtypes are unavailable. Current commercial assays have been shown to perform well with specimens from individuals infected with other HIV-1 subtypes, even group O ( 4, 23). Such devices could also be used to develop quantitative lateral-flow tests for a variety of diagnostic applications.įor rapid HIV testing, lateral-flow tests primarily use HIV-1 subtype B antigens from the immunodominant transmembrane region to capture HIV-specific antibodies. Diagnostic-instrument manufacturers have responded by developing lateral-flow strip readers that use reflectance, fluorescence, and magnetic measurements to provide a more precise and objective result ( 7, 14). Investigations to determine the sources of these problems have not identified any particular trait other than the subjective interpretation of the results ( 3). Although they are generally easy to interpret by visual inspection, there are reports of false-reactive devices, particularly in low-prevalence settings ( 6). The tests are rapid, inexpensive, and stable over a broad temperature range and are simple to perform, requiring no additional equipment ( 1). These assays are primarily designed as lateral-flow formats that use colored detection reagents, such as colloidal gold or selenium, conjugated to HIV antigens or to proteins that bind to specific human immunoglobulins, such as protein A or G ( 10). The use of rapid HIV antibody-screening assays has permitted the global expansion of HIV testing into rural, nonlaboratory settings and has significantly increased the number of individuals that have been screened. MICT can provide a rapid, low-cost method of determining HIV antibody status requiring no subjective interpretations. Assay reproducibility (observed MAR) for both intra- and interrun testing was excellent, with coefficients of variation of <12%. Additionally, 13 HIV-1 seroconversion panels (total specimens = 85), a worldwide panel containing seven of the major circulating HIV-1 subtypes ( n = 18), an HIV-2 panel, an HIV-1/HIV-2 mixed panel, and 100 prospective specimens were tested with completely concordant results. The panel was comprised of samples from individuals infected with various HIV-1 subtypes ( n = 234) or HIV-2 ( n = 65) and HIV-seronegative specimens ( n = 350). The results of the optimized MICT assay were compared to standard enzyme immunoassay (EIA) and Western blotting (WB) results using a blinded 649-member panel of specimens from the United States, Cameroon, and West Africa. Magnetic immunochromatography testing (MICT) uses a standard lateral-flow platform that incorporates magnetic-bead conjugates for quantitative measurement of the magnetic field distortion associated with the bound magnetic conjugate (reported as adjusted relative magnetic units ). A simplified lateral-flow assay for the detection of antibodies to HIV using magnetic-bead conjugates and multibranched peptides from both HIV-1 and HIV-2 was developed.
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![]() Users are not your personal search engine. If you're not willing to put in some initial effort to read and learn, don't expect any help.Do a search on this subreddit, including the Megathread first, so as to not be repetitive.By not reading it first, you decided that the time the moderators and members spent contributing to the wiki was beneath you. Any posts that are confusing, low quality, or lazy such as only saying Title in the body text, will be discarded.ĭon't post questions already answered.Quit spamming the same issues, contentious politics, & misinformation.Harassment of others, as well as insulting remarks & inappropriate behavior, will result in a permanent ban without warning.Do not encourage rule breaking by asking which specific title another person is looking for when they make a request.ĭon't harass others, spam, or submit low-quality posts. ![]() No, you may not ask for a specific pirated title (eg.No, you may not link to a specific pirated title ( eg.Yes, you can link to the top level domain of a site ( eg.No self-promotion of your own work is allowed unless you expressly receive permission from the moderators.ĭon't request or link to specific pirated titles.No trading or selling content, especially not for monetary services.No asking for content downloads, activation keys etc.No pleading for invites to scene sites, private torrent trackers, DDL forums or other sites with closed registrations.Any visual depiction of seeding / leeching ratio is acceptable, provided that it does not include specific titles.ĭon't request invites, trade, sell, or self-promote.Images that are too blurry or have a lot of unintelligible text will be deleted. So, first you need to go to your Firestick device and then. To download Popcorn Time on Firestick you will need to perform a little bit of setting up. Here is a step-by-step guide on how to download and install the Popcorn Time Firestick App. Comics, memes amd screenshots are acceptable, but should be original and of good quality. This is why it is so easy to get the program on your Firestick.Stream HD movies and TV shows with subtitles for free. This version is also compatible with Firestick and Android TV, and it’s the one we recommend. You can download the application directly from their website. There’s a TV version that has a customized interface, but we prefer the Android version. Install Popcorn Time on Firestick & Android in 5 minutes 2023. The focus on file-sharing, articles and discussions about moral dilemmas with unauthorized distribution, legal developments, challenges, and other related topics are all welcome. Downloads Internet Tools Popcorn Time 6.2.1 The latest and safest official 2023 version of Popcorn Time. Now, we need to download the apk file to install Popcorn Time.Posts must be related to the discussion of digital piracy ![]() This site uses Google AdSense to serve targeted advertisements. All opinions expressed herein are the views of the entity known as CyFishy Traveler, and are not the views of Linden Lab or Linden Research, Inc. Webpage contents are © CyFishy Traveler, 2010. The Legal Stuff: Second Life® and Linden Lab® are trademarks or registered trademarks of Linden Research, Inc. Just keep in mind that what you read here may not match what you see on your personal viewer. Linden Lab has instituted a Third Party Viewer policy that places certain requirements on viewers for them to be officially accepted, so you might do best to pick one of those out. A comphrehensive list can be found on the Second Life Wiki here. Since the source code has been generously put out to be tinkered with, there are a wide number of viewers available to access Second Life with. For simplicity, tutorials on this site will refer to the most recent versions of the SL Viewer (depending on how quickly I can get updates up!) This site was originally put together under the previous iteration of the official Second Life viewer but is in the process of updated to reflect the changes in Viewer 2, which can be downloaded at. ![]() For example, following the exact instructions to add a bookmark in Internet Explorer will just confuse you terribly if you're working in Safari. Viewers, much like browsers, do roughly the same job but may have slight variations in the exact instructions. The way inworld is through a program known as a viewer. ![]() ![]() This is useful for video producers who have to encode (render) their footage several times. ![]() Rendered videos have a relatively high file size, but their quality is almost lossless. Renders an MP4 video encoded with the x264 codec in High Quality. This is default Encoding Preset, as it exports a video with good quality and a relativly low file size. Renders an MP4 video encoded with the x264 codec in Default Quality. There are 7 Encoding Presets you can choose from: Note: If you simply want to render an MP4 video in good quality, don't change these settings. using a video editing software like Premiere), make sure to export it at a 2:1 aspect ratio - otherwise it will look strange in 360 degrees.īelow the Render Method Dropdown, there is another Dropdown Menu for Encoding Presets. Note: If you're re-rendering Equirectangular footage (e.g. To get a decent looking video, we recommend a resolution of 4320 * 2160. Please note that you should generally use a high resolution when exporting Equirectangular Videos, as the Video will be mapped onto a spherical surface in the Viewer you're using. Videos in Equirectangular Projection always need to have an Aspect Ratio of 2:1. So if you want a Full HD Stereoscopic Video, you have to enter 3840 * 1080. When using Stereoscopic Rendering, please note that the entered Width value is the final video's width.īecause Stereoscopic Videos have two frames next to each other, you therefore need to double the video width, You can enter any resolution there, but make sure to use reasonable values.Ĭommon Video Resolutions for Default Rendering are: To play your first Camera Path, click the Play Button next to the Keyframe Timeline.īy default, the Camera Path starts from the Cursor Position on the Keyframe Timeline.īy clicking while holding the Ctrl key ( Cmd key on Mac), you can however start from the beginning regardless of the Cursor Position.īelow the Render Method Dropdown, you can enter the desired Video Resolution (Width * Height). Do not add a Time Keyframe containing an earlier point in time after another Time Keyframe. Note: Travelling backwards in time is not supported during Camera Paths. Hint: By right-clicking a Time Keyframe on the Keyframe Timeline, you can jump to this Keyframe's Timestamp Value. Two Time Keyframes with the same Timestamp will freeze the Replay time for the duration of their interval. When playing the Camera Path, the Replay's time will interpolate between the set Time Keyframes in the Keyframes' intervals. You can set a Time Keyframe using the "Add Time Keyframe" button.Ī Time Keyframe initially contains the Replay's point in time when creating the Keyframe. Time Keyframes can be used to precisely control time travelling during a Camera Path.Įvery Time Keyframe represents a timestamp in the Replay itself. Troubleshootingĥ.1 No FFmpeg installation found 5.2 Crash while rendering 5.3 Unsupported Launchers 5.4 Compatibility with other Modsĥ.4.1 General information 5.4.2 Shaders Mod 5.4.3 Custom Main Menu 5.4.4 Tickrate Changer 5.4.5 LabyMod 5.4.6 OldAnimationsMod 5.4.7 Orange's 1.7 Animations 5.4.8 Sk1er Club: Patcher 5.4.9 Baritone 5.4.10 RandomPatches 5.4.11 Sodium 5.4.12 Resource Loader 5.4.13 LiteLoader Replayingģ.1.1 Basic Controls 3.1.2 Camera Roll 3.1.3 Spectating Entities 3.2 Controlling Timeģ.2.1 Replay Speed 3.2.2 Jumping in Time 3.3 Camera Pathsģ.3.1 Introduction 3.3.2 Keyframe Timeline 3.3.3 Position Keyframesģ.3.3.1 Path Interpolation 3.3.3.2 Path Preview 3.3.4 Time Keyframesģ.3.4.1 Synchronizing Timelines 3.3.5 Spectator Keyframes 3.3.6 Editing Keyframes 3.3.7 Removing Keyframes 3.3.8 Keyframe Repository 3.4 Renderingģ.4.1.1 Default Rendering 3.4.1.2 Stereoscopic Rendering 3.4.1.3 Cubic Rendering 3.4.1.4 Equirectangular Rendering 3.4.2 Video Settingsģ.4.2.1 Video Resolution 3.4.2.2 Video Framerate 3.4.2.3 Video Encoding and Quality 3.4.3 Advanced Settingsģ.4.3.1 Render Nametags 3.4.3.2 Stabilize Camera 3.4.3.3 Chroma Keying 3.4.3.4 Depth Map 3.4.4 Command Line Settingsģ.4.4.1 Custom Command 3.4.4.2 Command Line Arguments 3.4.5 Rendering Performance 3.5 Ambient Lighting 3.6 Quick Mode 3.7 Player Overview 3.8 Thumbnails 3.9 Event Markers 4. Subsequent updates may yet change what updates are available and what combinations work, among other things, but the below list details all 580 elements featured in the game’s major August, 2017 update.1.1 Installing the Replay Mod 1.2 Installing FFmpegġ.2.1 Windows 1.2.2 Mac OSX 1.2.3 Linux 1.3 Settings 1.4 Accounts 2. Little Alchemy has been updated since its release, and the total available as of April of 2018 is 580. Play Complete Element and Ingredient Combination List for Little Alchemy The most recent example of this was the 2015 NLCS, when the 90-win, division-winning Mets had home field advantage over the 97-win, Wild Card Cubs. Home field advantage goes to the team with the best record, though a Wild Card winner cannot have home field advantage in the League Championship Series. The League Championship Series and World Series are both best-of-seven formats in a 2-3-2 schedule. The Division Series are best-of-five with a 2-2-1 format. 2, followed by the American League on Wednesday, Oct. Hyperbole Not when you put what they are doing into. Ranking the possible 2018 World Series matchups Teamsīoth Wild Card Games are single-elimination, with the National League starting things off on Tuesday, Oct. 28 seconds ago In this story: Atlanta Braves It’s time to start talking about the Braves’ offense as the best since the Big Red Machine. Please note that all dates, times and opponents are subject to change. Other playoff droughts erased are the young Atlanta Braves (last playoffs: 2013) and the surging Oakland Athletics (2014). Cubs Printable Schedule Print your Chicago Cubs regular season schedules. The Milwaukee Brewers are back in the playoffs for the first time since 2011 and are the top seed in the National League. Seven teams from last year’s playoffs are back, including both World Series participants - the Houston Astros and Los Angeles Dodgers. ![]() They were joined by the Houston Astros (103 wins) and the New York Yankees (100), the first time that there have been three 100-win teams in the same league. baseball in the Dominican Republic around 1937 (MLB.com/DR 2018). 1, but now that the National League is all settled we get the real thing, with four weeks of playoff baseball to entertain us for the remainder of October.įor the second season in a row we have three 100-win teams, lead by a juggernaut Boston Red Sox team that set a franchise record with 108 victories. In fact, some of the greatest Major League Baseball players of all-time like Roberto. The Cubs were managed by Joe Maddon, in his fourth year as Cubs manager, and played their home games at Wrigley Field as members of Major League Baseball 's National League Central Division. Baylor, Don, 68, baseball player who in 19 MLB seasons won the AL MVP (1979) and a World Series (1987) later managed Colorado and Chicago Cubs Austin, TX. We got a nice head start on postseason baseball with the unprecedented double tiebreaker on Oct. The 2018 Chicago Cubs season was the 147th season of the Chicago Cubs franchise, the 143rd in the National League and the Cubs' 103rd season at Wrigley Field. ![]() ![]() Game 5: Red Sox 5, Dodgers 1 Postseason preview David Price set his postseason narrative on fire and buried the ashes in Los Angeles Steve Pearce wins World Series MVP World Series results ![]() The existing 2.7-mile loop through downtown Atlanta gets about 158,000 riders per year. If the current Atlanta Streetcar is any indication, most of the operating costs for the extension will be covered by people who never ride it. The project is being funded by a half-cent increase in the Atlanta sales tax, and the extension is scheduled to open in 2028.Įven if the extension doesn't go over budget, $230 million for two miles of new streetcar track works out to a slobber-knocking total of $21,700 per foot.Īnd that only covers the construction costs. The Center Square reports that the first batch of that new spending-an $11.5 million contract awarded to a firm that will design the extension-was doled out last week. The Metropolitan Atlanta Rapid Transit Authority (MARTA) is moving ahead with plans to build a 2-mile extension to the city's 2.7-mile streetcar line, with an estimated price tag of $230 million. Every single person who was at that train station was different,” Charlotte said.What is already arguably America's worst public transit project is about to get a whole lot more expensive. “We probably won’t get two pairs of the exact same. She hopes the shoes will remind people of the individual toll of war. “I know a lot of students have come up to me and said they weren’t aware of the missile hitting the train station before we started raising awareness.” “It’s just crazy to think that something could happen in something so normal or something so pedestrian as a train station,” Charlotte said. The images and stories from Russia’s invasion of Ukraine hit home. Explore More stories about Atlanta Public Schools As part of their work on the debate team, she and Sierra researched the threat Russia posed and NATO’s role in the region. MARTA spokeswoman Stephany Fisher said the transit agency is pleased to be working with the students.Ĭharlotte Keenan, a 15-year-old Midtown High School freshman, is Sierra’s debate partner and a member of the committee organizing the event. “The hope for this is that it gives an avenue for people our age to get involved in this issue.” “As students, we don’t really know how to help in situations like this directly,” Sierra said. Explore Atlanta’s Midtown High School unveils new logos after Grady name change A Midtown High School sophomore who was born in the Ukraine also will speak. School orchestra members will play the “State Anthem of Ukraine.” They’ll hold a moment of silence, and speakers will talk about relief efforts and ways to support the people of Ukraine. Afterward, the footwear will be donated to a charity that will give them to needy men, women and children in Atlanta. ![]() The shoes will be displayed in the Atlanta train station to symbolize each Ukrainian killed or wounded in the attack. A post shared by Atlanta Students for Ukraine students set out bins in their high school hallways and a nearby bakery with the goal of collecting 148 pairs of shoes from classmates, neighbors, dance studios and churches. Ardour was written to run on operating systems that properly and efficiently support a portable operating system standard called POSIX (endorsed by the US government and many other large organizations). The obstacles are relatively few in number, but rather substantial in significance. There have been several discussions about porting Ardour to Windows. Also, what you said about their being no ardour for windows just isn’t true. I actually pay for the Windows software I use and rely on. 95% of windows crashes are due to poorly coded 3rd party software, dont you know. Microsoft still stands because, aside from weak default security settings, they have a reliable OS. Making open source apps run on windoze also adds buoyancy to that rats nest too, and it’s great that Ardour will not support it.” The only reason microsoft still stands as a company is because of piracy… and if everyone had to actually pay for their software that ship would sink quicker than the Titanic. “Actually I wish none of the Linux FOSS apps were ported to windoze. How rude! Now lets take apart what you just said. Do yourself a favor and make the jump from windoze to Linux and don’t look back, because it’s a lot easier to install Linux on a PC (Ubuntu Studio has Ardour pre-installed in an ISO, for example) than it is to code the changes need to compile Ardour for windoze. Microsoft is already giving away XP to some favored PC builders, and the absolute low-end CPU’s are plenty powerful for running Linux, so the clock is ticking on their demise. But that’s not the only pressure on microsoft: when the price of PC hardware becomes obscenely cheap, commodity prices below $200, windoze will be priced out of the equation. There was even a movement inside the warez community which advocated, “no crack for vista.” It seemed odd that for months there was no crack and only feeble attempts like resetting the trial period clock, and then a new warez group emerged with a legitimate crack, some claim it came from microsoft itself. ![]() There have been many many arrests over the last few years and some piracy groups have outright quit. One funny footnote to this is that the warez scene is dying. Congrats on pirating VisualStudio, it shows that you should have no problem pirating Sonar, Cubase, or dozens of smaller sequencers. That is so naive that I feel like hitting you with a stick. ![]() You seem to think it’s as simple as adding a library/API, compling, and poof! it’s done. Now to the possibility of a port: for you, zero. Making open source apps run on windoze also adds buoyancy to that rats nest too, and it’s great that Ardour will not support it. I wish none of the Linux FOSS apps were ported to windoze. Roblox promo codes are a perfect way to save yourself some Robux. Roblox animation and emotes Roblox bundles Items Roblox free heads Roblox free faces Free faces It depends upon the type of Code you have redeemed. You will get Credit, Robux, or a Virtual item. Roblox free hair Roblox free heads Free Heads A success message will appear once you've successfully redeemed the Code. Make your avatar look shining with these items: Roblox free hair Items The following list features free items you can find in the catalog. The following list consists of previous codes in the game but no longer work. Unfortunately, Roblox promo codes expire after some time. RewardsĪ free Crystalline Companion (Must be redeemed in Island of Move game)Ī free Kinetic Staff (Must be redeemed in Island of Move game)Ī free Build it Backpack (Must be redeemed in Island of Move game)Ī free Hustle Hat (Must be redeemed in Island of Move game)įree Speedy Shades (Must be redeemed in Island of Move game)įree Cardio Cans (Must be redeemed in Island of Move game) Here is a list of the latest active All Roblox promo codes that work for October 2020 and what you can get when redeeming them. Note that promo code may expire or only be active for a short time, so make sure to use your code immediately. ![]() If there is an unknown and unspecified error, this shows up:Īfter you’ve entered a code and redeemed, your free virtual good will be automatically added to your Roblox account. If you tried to redeem a code without being logged in, this shows up: If you have already redeemed the promo code, this shows up: If the promo code is expired or invalid, this shows up: If you redeemed it successfully, this shows up: We’ll keep this list updated so that you can view it on the go.
![]() ![]() Photo: Denver Post via Getty Images This year marks 50 years for the Eisenhower tunnel, the first route through the Continental Divide, connecting the state along Interstate 70.Weather in Eisenhower Memorial Tunnel (Colorado). Because of the gneiss mica rock, only 8 feet could be blasted at one time. 10, 1960: The south Eisenhower Tunnel included a steel lining that slowed blasting progress. Snow, wind move through near Eisenhower Tunnel News / / 07:56 AM MST Snow, wind move through near Eisenhower Tunnel, Jim Hooley reports Clear Creek water restrictions lifted.In Colorado, it provides major access to the western slope of the Continental Divide. It is the longest (1.7 miles) mountain tunnel and the highest point (11,158’) on the Interstate Highway System. The Eisenhower Tunnel (west bore), and Johnson Memorial Tunnel (east bore) (map), is one of the highest vehicular tunnels in the world. The tunnel is the longest mountain tunnel and highest. With a maximum elevation of 11,158 ft (3,401 m), it is the highest vehicular tunnel in the world. It carries Interstate 70 under the Continental Divide in the Rocky Mountains. ![]() The tunnel is approximately 60 mi (97 km) west of Denver, Colorado. ABS EVENING NEWS (SATURDAY 29TH JULY 2023) Eisenhower/Johnson Memorial Tunnel, Colorado. Eastbound I-70 closed at Genesee due to crashes around 12:15 p.m. When you think of “fire department,” you think about a service run by a city to provide first response in case of fires, and while that may not be the case for the Eisenhower Tunnel, engineers who built the tunnel put in place a plan to protect people in case of a fire inside.Shortly before 9 p.m., the westbound lanes of the interstate fully reopened between Golden and the Eisenhower Tunnel. The tunnel has its own “fire department” of sorts. Learn about CDOT services and tools available to ease your transportation needs. |
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